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Related Experiment Videos

Prolactin receptor expression by rat NK cells

W H Chambers1, A A Amoscato, M S Smith

  • 1University of Pittsburgh Cancer Institute, Pennsylvania, USA.

Natural Immunity
|January 1, 1995
PubMed
Summary

Prolactin (PRL) affects rat natural killer (NK) cells. Researchers found prolactin receptors (PRLr) on NK cells, indicating PRL may influence their function.

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Area of Science:

  • Immunology
  • Endocrinology
  • Cell Biology

Background:

  • Prolactin (PRL) is a hormone known to influence immune cell function.
  • Natural killer (NK) cells are crucial for innate immunity.
  • The role of PRL in NK cell biology is not well understood.

Purpose of the Study:

  • To investigate the presence and regulation of prolactin receptors (PRLr) on rat NK cells.
  • To determine the effects of interleukin-2 (IL-2) and PRL on NK cell PRLr expression.
  • To explore the potential functional implications of PRL-PRLr interaction in NK cells.

Main Methods:

  • Reverse transcription/polymerase chain reaction (RT/PCR) and Southern blotting to detect PRLr transcripts.
  • Flow cytometry and 251-PRL binding assays to quantify PRLr expression.
  • Analysis of NK cells from normal, IL-2-treated, and bromocriptine-treated rats.

Main Results:

  • A PRLr specific transcript was identified in IL-2-activated NK (A-NK) cells, showing homology to rat liver PRLr.
  • A-NK cells expressed detectable intracellular PRLr, but not surface PRLr.
  • Surface PRLr was detected on NK cells from bromocriptine-treated rats, suggesting regulation by PRL levels.

Conclusions:

  • Rat NK cells express prolactin receptors (PRLr), both in cultured and freshly isolated states.
  • IL-2 does not induce detectable surface PRLr on NK cells.
  • PRLr expression on NK cells suggests a potential role for prolactin in regulating NK cell function.

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