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Tween 80 and endothelium: functional reduction due to tissue damage
C Uluoglu1, P Korkusuz, O Uluoglu
1Gazi University, Medical School, Department of Pharmacology, Ankara, Turkey.
Research Communications in Molecular Pathology and Pharmacology
|February 1, 1996
Summary
High concentrations of Tween 80 disrupt endothelial function by damaging the aortic lining, inhibiting endothelium-derived relaxing factor (EDRF) release. Moderate concentrations showed no significant effects.
Area of Science:
- Pharmacology
- Endothelial Biology
- Cardiovascular Research
Background:
- Tween 80 is a common surfactant used to disperse water-insoluble substances.
- Endothelial function is crucial for vascular health, involving the release of factors like EDRF.
- The impact of Tween 80 on endothelial function requires detailed investigation.
Purpose of the Study:
- To investigate the effects of Tween 80 on endothelial function in rabbit aorta.
- To determine the mechanism by which Tween 80 might affect endothelium-derived relaxing factor (EDRF) release.
Main Methods:
- A cascade superfusion bioassay system was employed.
- Deendothelialized, precontracted rat aorta rings served as bioassay tissue.
- Rabbit thoracic aorta segments were pre-incubated and perfused with varying concentrations of Tween 80.
Main Results:
- Higher concentrations of Tween 80 completely inhibited acetylcholine-induced EDRF release.
- Moderate Tween 80 concentrations did not significantly affect EDRF release.
- Perfusion with Tween 80 inhibited EDRF release in a dose-dependent manner.
- Histopathology revealed significant endothelial desquamation at the highest Tween 80 concentration.
Conclusions:
- Tween 80, particularly at higher concentrations, can destroy the endothelial lining of blood vessels.
- The observed reduction in EDRF release is likely due to Tween 80-induced damage to the endothelium.