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An in vitro microbial model for studying secondary caries formation
M Fontana1, A J Dunipace, R L Gregory
1Oral Health Research Institute, Indianapolis, Ind., USA.
Caries Research
|January 1, 1996
Summary
A new in vitro model using specific oral bacteria and sucrose successfully created secondary caries lesions on composite restorations. This model aids in studying the causes and prevention of secondary caries.
Area of Science:
- Dental research
- Microbiology
- Biomaterials science
Background:
- Secondary caries is a primary cause for dental restoration replacement.
- Pathogenic oral bacteria are hypothesized to be closely linked to secondary caries development.
Purpose of the Study:
- To develop and validate an in vitro microbial model for producing secondary carious lesions.
- To investigate the microbial etiology of secondary caries.
Main Methods:
- An in vitro model utilized a mixture of Streptococcus mutans and Lactobacillus casei with sucrose.
- Human tooth specimens with composite restorations were exposed to cyclic challenges of the bacterial inoculum and a mineral wash solution.
- Laser scanning confocal microscopy was employed to assess lesion development.
Main Results:
- The experimental model successfully induced incipient surface and wall lesions in composite restorations within 7 days.
- The pH in experimental groups dropped significantly (4.1-4.5), while control groups maintained a neutral pH (6.8-7.0).
- Bacterial viability was confirmed, and the model's reproducibility was established.
Conclusions:
- The developed in vitro model effectively simulates secondary caries formation.
- This model is suitable for studying the microbial causes and potential prevention strategies for secondary caries.