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Related Experiment Videos

Gene transfer to neurons using herpes simplex virus-based vectors

D J Fink1, N A DeLuca, W F Goins

  • 1Department of Neurology University of Pittsburgh, Pennsylvania 15261, USA.

Annual Review of Neuroscience
|January 1, 1996
PubMed
Summary

Herpes simplex virus type 1 (HSV-1) shows promise as a gene therapy vector for the nervous system. Researchers are developing safe and efficient HSV-1 vectors for direct in vivo gene transfer to neurons.

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Area of Science:

  • Molecular Medicine
  • Neuroscience
  • Gene Therapy

Background:

  • Gene transfer technologies are crucial for molecular medicine and clinical practice.
  • Developing safe and efficient gene delivery vectors for specific tissues, especially postmitotic neurons, remains a challenge.
  • Herpes simplex virus type 1 (HSV-1) is a neurotropic virus that naturally infects neurons and establishes latency, suggesting its potential as a gene transfer vector.

Purpose of the Study:

  • To review the suitability of HSV-1 as a gene transfer vector for the nervous system.
  • To discuss strategies for modifying HSV-1 to reduce pathogenesis and enhance transgene expression.
  • To evaluate the efficiency and safety of HSV-1-based vectors in gene transfer experiments.

Main Methods:

  • Review of HSV-1 molecular biology.

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  • Description of strategies for recombinant vector safety enhancement.
  • Methods for expressing transgenes from engineered HSV-1 genomes.
  • Evaluation of gene transfer experiments using recombinant and amplicon HSV-1 vectors.

    • Main Results:

    • HSV-1 possesses unique characteristics making it a viable candidate for neural gene delivery.
    • Recombinant and defective HSV-1 vectors have been developed and tested for gene transfer.
    • The efficiency and safety of these HSV-1-based vectors in gene transfer applications have been assessed.

    Conclusions:

    • HSV-1-based vectors offer a promising approach for gene therapy in the nervous system.
    • Further development is needed to optimize safety and efficiency for clinical applications.
    • HSV-1 vectors hold potential for revolutionizing experimental neuroscience and clinical medicine.