Determination of the pharmaceutical peptide TP9201 by post-column reaction with copper(II) followed by...

An electrochemical detection method was applied to the determination of the synthetic peptide TP9201 (Telios Pharmaceuticals). The method utilizes reversed-phase HPLC, followed by post-column formation of Cu(II)-peptide complexes to render peptides electrochemically active via the Cu(III/II) couple. TP9201 is cyclic and N-amidated; the lack of a free amine precludes the use of typical fluorescent labeling reagents. Neither the cyclic structure nor the N-amidation prevented the copper complexation reaction, however. The detection limit in bovine serum was 20 nM, limited by interfering sample peaks, and the detector response was linear in a range 10-400 nM.