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A high-resolution map around the locus Om on mouse Chromosome 11
P A Baldacci1, M Cohen-Tannoudji, C Kress
1Unité de Biologie du Développement, U.R.A.C.N.R.S. 1960, Institut Pasteur, Paris, France.
Summary
The mouse Om (ovum mutant) locus affects early embryo viability. This study precisely maps the Om locus on Chromosome 11, aiding its future molecular characterization.
Area of Science:
- Genetics
- Developmental Biology
- Reproductive Biology
Background:
- The mouse locus Om (ovum mutant) is known to impact the viability of preimplantation embryos resulting from crosses involving the DDK strain.
- Previous research localized the Om locus to Chromosome 11, in proximity to the Scya2 gene.
Purpose of the Study:
- To generate a high-resolution genetic map of the region surrounding the Om locus.
- To refine the physical boundaries of the Om mutation for positional cloning efforts.
Main Methods:
- High-resolution mapping using a large cohort of backcross individuals.
- Comparative mapping analysis with data from the EUCIB backcross.
Main Results:
- The Om locus was precisely delimited on Chromosome 11, with Scya2 defining the proximal boundary and D11Mit36 defining the distal boundary.
- The genetic distance between Scya2 and D11Mit36 was estimated to be approximately 2 centimorgans (cM).
Conclusions:
- The refined genetic map provides precise boundaries for the Om mutation.
- These findings are crucial for facilitating the positional cloning and subsequent molecular characterization of the Om gene.