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Related Experiment Videos

Gene encoding human Ro-associated autoantigen Y5 RNA

R Maraia1, A L Sakulich, E Brinkmann

  • 1Laboratory of Molecular Growth Regulation, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA.

Nucleic Acids Research
|September 15, 1996
PubMed
Summary
This summary is machine-generated.

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The human Y5 RNA gene, a component of Ro ribonucleoproteins, was isolated and characterized. Phylogenetic analysis reveals positive selection during primate evolution, suggesting a retroposon origin.

Area of Science:

  • Molecular Biology
  • Genetics
  • Evolutionary Biology

Background:

  • Ro ribonucleoproteins (RNPs) consist of Y RNAs and the Ro 60 kDa protein.
  • The function of Y RNAs is unknown, though Y5 RNA is a major component of human Ro RNPs and an autoimmune antigen.
  • Isolation of Y RNA genes is challenging due to numerous retroposed pseudogenes.

Purpose of the Study:

  • To isolate and characterize the source gene for Y5 RNA.
  • To determine the evolutionary history and genomic location of the Y5 RNA gene.
  • To compare the activity of the Y5 RNA gene with other Y RNA genes.

Main Methods:

  • Isolation of the Y5 RNA source gene from human and Galago senegalis DNA.
  • In vivo demonstration of hY5 RNA gene authenticity and comparison with hY4 RNA gene activity.

Related Experiment Videos

  • Determination of the linear order of human Y RNA genes on chromosome 7q36 using comparative genomic analyses.

    • Main Results:

    • The source gene for Y5 RNA was successfully isolated from human and primate DNA.
    • The hY5 RNA gene demonstrated activity in vivo and utilizes a type 3 promoter for RNA polymerase III, similar to hY4 RNA.
    • The hY5 RNA gene is located near other Y RNA genes on chromosome 7q36, and phylogenetic analyses indicate positive selection during primate evolution, supporting a retroposon origin.

    Conclusions:

    • The Y5 RNA gene has been identified and characterized, providing insights into its function and evolution.
    • Evidence suggests the Y5 RNA gene originated as a retroposon and has undergone positive selection in primates.
    • The genomic organization and evolutionary trajectory of Y RNA genes are elucidated.