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Bovine immunoglobulin G subclass receptor sites on bovine macrophages
Macrophage cultures were initiated from bovine peripheral blood monocytes. The following antiserums were prepared: rabbit immunoglobulin (Ig) M anti-sheep erythrocyte (RBC) serum, bovine IgG1 anti-rabbit Ig serum, and bovine IgG2 anti-rabbit Ig serum. Erythrocytes treated singly with any of these serums failed to mediate attachment of RBC to macrophages. Erythrocytes treated with rabbit IgM anti-sheep RBC serum followed by either bovine IgG 1 or IgG2 anti-rabbit Ig serum attached to bovine macrophages, as seen microscopically. Results indicate that bovine macrophages contain receptors for both bovine IgG subclasses.
Macrophage cultures were initiated from bovine peripheral blood monocytes. The following antiserums were prepared: rabbit immunoglobulin (Ig) M anti-sheep erythrocyte (RBC) serum, bovine IgG1 anti-rabbit Ig serum, and bovine IgG2 anti-rabbit Ig serum. Erythrocytes treated singly with any of these serums failed to mediate attachment of RBC to macrophages. Erythrocytes treated with rabbit IgM anti-sheep RBC serum followed by either bovine IgG 1 or IgG2 anti-rabbit Ig serum attached to bovine macrophages, as seen microscopically. Results indicate that bovine macrophages contain receptors for both bovine IgG subclasses.