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Related Experiment Videos

An economic approach to the MIC

C Chapuis1, M L Delignette-Muller, J P Flandrois

  • 1Laboratoire de bactériologie, Centre hospitalier Lyon-Sud, Pierre Bénite, France.

Zentralblatt Fur Bakteriologie : International Journal of Medical Microbiology
|June 1, 1996
PubMed
Summary

A new method called Inhibitory Concentration in Diffusion (ICD) offers a faster alternative to Minimal Inhibitory Concentration (MIC) testing. This technique accurately measures antibiotic effectiveness, saving time and resources in clinical labs.

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Area of Science:

  • Clinical Microbiology
  • Antimicrobial Susceptibility Testing
  • Pharmacology

Background:

  • Agar dilution Minimal Inhibitory Concentration (MIC) is time-consuming and cumbersome for routine clinical use.
  • Disk diffusion tests are widely used but typically yield qualitative results.

Purpose of the Study:

  • To propose and validate the Inhibitory Concentration in Diffusion (ICD) as a rapid, cost-effective alternative to MIC testing.
  • To assess the correlation and reproducibility of ICD compared to conventional MIC methods.

Main Methods:

  • The ICD method was developed based on disk diffusion, calculating antibiotic concentration at the inhibition zone edge.
  • Antibiotics (ampicillin, cefotaxime, erythromycin, gentamicin, nalidixic acid, rifampicin, cefsulodin, ceftazidime, imipenem, piperacillin, ticarcillin, tobramycin) were tested against various bacterial strains (Enterobacteria, S. aureus, Enterococcus spp., P. aeruginosa).
  • ICD was calculated using inhibition zones from full, half, and quarter antibiotic disks; MIC was determined concurrently using agar dilution.

Main Results:

  • A good correlation was observed between the Inhibitory Concentration in Diffusion (ICD) and Minimal Inhibitory Concentration (MIC) methods.
  • The ICD technique demonstrated correct reproducibility across multiple antibiotic-strain combinations.
  • The method proved efficient in terms of laboratory time and material costs.

Conclusions:

  • The Inhibitory Concentration in Diffusion (ICD) is a reliable and efficient alternative to MIC testing for routine clinical laboratory use.
  • This novel technique offers significant advantages in speed and cost-effectiveness for antimicrobial susceptibility testing.
  • ICD has the potential for widespread adoption in clinical settings.

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