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Expression and regulation of prostaglandin F receptor mRNA in rodent osteoblastic cells
K Nemoto1, P M Bernecker, C C Pilbeam
1Department of Medicine, University of Connecticut Health Center, Farmington, USA.
Prostaglandins
|November 1, 1995
Abstract:
The ability of prostaglandins to inhibit collagen synthesis and induce prostaglandin G/H synthase in bone cells appears to be mediated by the prostaglandin F2 alpha receptor (FPR). We have identified FPR mRNA in the osteoblastic cell lines, Py1a from rats and MC3T3-E1 from mice, as well as in the stem cell cultures, MN-7 and mouse marrow, using reverse transcriptase-polymerase chain reaction technology (RT-PCR). Expression of FPR mRNA was increased in Pyla, MN-7 and marrow cells with prolonged culture or dexamethasone treatment and decreased after treatment with fluprostenol, a selective FPR agonist.