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Related Experiment Videos

Glycoconjugates in normal and abnormal secondary neurulation

C M Griffith1, T Hsieh, C Smith

  • 1Department of Anatomy and Neurobiology, University of Ottawa, Ontario, Canada.

Teratology
|November 1, 1995
PubMed
Summary

Cell surface glycoconjugates, specifically those binding Datura stramonium agglutinin (DSA), are crucial for secondary neural tube development in chick embryos. Disrupting these molecules causes neural tube defects, highlighting their role in cell differentiation.

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Area of Science:

  • Developmental Biology
  • Glycobiology
  • Neuroscience

Background:

  • The secondary neural tube forms from tail bud mesenchyme undergoing mesenchymal-epithelial transformation.
  • Cell surface glycoconjugates are implicated in tissue differentiation processes.

Purpose of the Study:

  • To investigate the role of cell surface glycoconjugates in the development of the secondary neural tube from chick embryo tail bud mesenchyme.
  • To identify specific glycoconjugates involved in this differentiation process.

Main Methods:

  • Lectin histochemistry and blotting using Datura stramonium agglutinin (DSA), which binds N-acetylglucosamine oligomers.
  • Ultrastructural lectin cytochemistry to localize DSA binding.
  • Teratogenic induction of neural tube defects using DSA and retinoic acid.

Related Experiment Videos

  • TUNEL assay to assess cell death.

    • Main Results:

    • DSA preferentially binds to differentiating tail bud cells, indicating specific glycoconjugate involvement.
    • DSA teratogenesis causes neural tube and notochord defects, independent of cell death.
    • Retinoic acid treatment perturbs DSA-binding patterns.

    Conclusions:

    • Cell surface glycoconjugates, particularly those recognized by DSA, are essential for normal secondary neural tube formation.
    • Aberrant glycoconjugate activity leads to developmental defects in the secondary neural tube and associated structures.