Different action of hormonal stimulation on the biosynthesis of three pancreatic enzymes

The rates of biosynthesis of amylase, lipase and chymotrypsinogen were followed in rat pancreas in vivo after intravenous injection of the hormone cholecystokinin-pancreozymin (8 IDU/kg) associated with secretin (5 CU/kg). This pancreatic stimulation resulted in non-parallel variations of the rates of biosynthesis of the three studied enzymes, suggesting independent regulation of synthesis. The result of one stimulation was calculated in terms of quantities of enzymes synthesized by the pancreas in comparison to control. It was found that chymotrypsinogen, amylase and lipase productions were increased by 63, 26 and 10%, respectively, indicating that repeated cholecystokinin-pancreozymin plus secretin stimulations could induce "adaptation-like" modifications of the pancreatic enzyme content.