Essential fatty acid metabolism in cultured astroblasts

The fetal calf serum on which two astroblast cell lines were grown was shown to be deficient in essential fatty acids. The fatty acid profiles of lipids of these two cell lines showed very low amounts of polyunsaturated fatty acids. In order to know if this low level of unsaturation was due to the lack of essential fatty acids in serum, or to a lack of desaturase activities in the cells, we have investigated the modifications of the cell lipid fatty acid patterns when serum was enriched in essential fatty acids. Linoleic acid was incorporated in rather high amounts in the cell lipids, while linolenic acid was very poorly incorporated. These two essential fatty acids were converted into polyunsaturated fatty acids only when they were added alone to the serum. Both cell clones exhibited a lack in the delta 4 desaturase activity. No morphological changes of the cells occured after nine days of culture with modified serum.