Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

E2F-induced S phase requires cyclin E

R J Duronio1, A Brook, N Dyson

  • 1Department of Biochemistry and Biophysics, University of California, San Francisco 94143, USA.

Genes & Development
|October 1, 1996
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Patient experiences in trials of minimally invasive thoracic surgery: A mixed-methods study.

Journal of robotic surgery·2026
Same author

Adapting the MPOWER policy framework for fossil fuels and public health: reflections on content and process.

Perspectives in public health·2025
Same author

Fiscal and monetary policies: the cutting edge of advocacy and research on population health and climate change.

Perspectives in public health·2021
Same author

The Impact of COVID-19 on Emergent Large-Vessel Occlusion: Delayed Presentation Confirmed by ASPECTS.

AJNR. American journal of neuroradiology·2020
Same author

COVID-19-Associated Carotid Atherothrombosis and Stroke.

AJNR. American journal of neuroradiology·2020
Same author

Diagnostic Utility of Optic Nerve Measurements with MRI in Patients with Optic Nerve Atrophy.

AJNR. American journal of neuroradiology·2019
Same journal

Corrigendum: Inhibition of Myc family proteins eradicates KRas-driven lung cancer in mice.

Genes & development·2026
Same journal

A new perspective on ATR's role in translesion synthesis.

Genes & development·2026
Same journal

Mechanisms coordinating exit from the stem cell state in mammals.

Genes & development·2026
Same journal

Evolutionarily conserved spliceosome-exosome pathway in nuclear mRNA surveillance.

Genes & development·2026
Same journal

CDK1 and CEP97 cooperatively control centriole length to orchestrate ciliogenesis and developmental patterning.

Genes & development·2026
Same journal

Coupling of translesion synthesis with the replisome stabilized at stalled replication forks by ATR.

Genes & development·2026
See all related articles

Cyclin E is crucial for DNA replication and the cell cycle

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Genetics

Background:

  • The G1-S transition in the cell cycle requires both E2F transcription factors and G1 cyclins, like cyclin E.
  • Cyclin E is known to activate E2F, acting upstream in this process.

Purpose of the Study:

  • To investigate the role of cyclin E in DNA replication beyond its function in activating E2F.
  • To determine if E2F-directed transcription can overcome the absence of cyclin E during the cell cycle.

Main Methods:

  • Generated transgenic Drosophila with inducible, ectopic E2F activity.
  • Overexpressed both Drosophila E2F subunits (dE2F and dDP) in embryos.
  • Utilized cyclin E mutations to assess the necessity of cyclin E for E2F-driven S phase initiation.

Related Experiment Videos

Main Results:

  • Overexpression of dE2F/dDP induced expression of E2F target genes, including cyclin E, and initiated S phase.
  • Loss of cyclin E function prevented S phase initiation upon dE2F/dDP overexpression.
  • E2F target gene induction proceeded normally even when cyclin E was mutated.

Conclusions:

  • Cyclin E plays an essential, distinct role in DNA replication separate from E2F activation.
  • E2F-mediated transcription cannot bypass the requirement for cyclin E in initiating DNA replication and S phase.
  • This highlights a critical, non-redundant function of cyclin E in cell cycle progression.