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Gentamicin concentrations in human subcutaneous tissue

H Lorentzen1, F Kallehave, H J Kolmos

  • 1Copenhagen Wound Healing Center, Bispebjerg University Hospital, Denmark.

Antimicrobial Agents and Chemotherapy
|August 1, 1996
PubMed
Summary
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Gentamicin concentrations in subcutaneous tissue were measured using microdialysis. The study found sufficient gentamicin levels in adipose tissue to combat common bacteria, suggesting effective wound infection treatment.

Area of Science:

  • Pharmacology
  • Wound Healing
  • Infectious Diseases

Background:

  • Subcutaneous tissue is a common origin for wound infections.
  • Evaluating gentamicin's efficacy typically relies on blood or wound fluid concentrations, not direct tissue measurement.

Purpose of the Study:

  • To investigate the pharmacokinetic properties of gentamicin in human subcutaneous adipose tissue.
  • To assess gentamicin concentrations in subcutaneous tissue using microdialysis.

Main Methods:

  • Seven healthy volunteers received a 240-mg intravenous gentamicin bolus.
  • Microdialysis probes were inserted into the abdominal subcutaneous fat layer.
  • Simultaneous measurements of gentamicin in serum and subcutaneous interstitial fluid were taken for 6 hours.

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Main Results:

  • Gentamicin tissue concentration peaked between 10-30 minutes, reaching 39.1% of peak serum concentration.
  • The area under the concentration-time curve in tissue was 59.7% of that in serum over 6 hours.
  • Peak gentamicin tissue concentrations were 7x the MIC for Pseudomonas aeruginosa and 33x for Staphylococcus aureus.

Conclusions:

  • Microdialysis is a viable technique for dynamic, quantitative measurement of gentamicin in subcutaneous tissue.
  • The study demonstrated adequate gentamicin concentrations in adipose tissue for effective antibacterial action against common pathogens.
  • Findings support the potential for effective gentamicin treatment of subcutaneous wound infections.