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Apolipoprotein J expression in human ocular surface epithelium

K Nishida1, S Kawasaki, W Adachi

  • 1Department of Ophthalmology, Kyoto Prefectural University of Medicine, Japan.

Investigative Ophthalmology & Visual Science
|October 1, 1996
PubMed
Summary

Apolipoprotein J (apoJ) is expressed in the human ocular surface epithelium. This protein is synthesized by and localized within corneal and conjunctival cells, suggesting a role at the tear-ocular surface interface.

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Area of Science:

  • Ocular surface biology
  • Epithelial cell research
  • Protein expression studies

Background:

  • Apolipoprotein J (apoJ) expression is noted in various mucosal epithelial cells and ocular tissues.
  • Previous studies indicate apoJ presence in the human ciliary body, retina, vitreous, and aqueous humor.

Purpose of the Study:

  • To investigate the expression and localization patterns of apoJ mRNA and protein.
  • Focus on the human ocular surface epithelium, specifically corneal and conjunctival cells.

Main Methods:

  • Reverse-transcriptase polymerase chain reaction (RT-PCR) for apoJ mRNA detection.
  • In situ hybridization for mRNA localization within the corneal epithelium.
  • Immunohistochemistry for apoJ protein localization in corneal and conjunctival epithelia.

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Main Results:

  • RT-PCR confirmed apoJ mRNA expression in corneal and conjunctival epithelial cells.
  • In situ hybridization showed apoJ mRNA in all corneal epithelium layers, primarily basal cells.
  • Immunohistochemistry revealed apoJ protein in the apical layers of corneal and conjunctival epithelia.

Conclusions:

  • Apolipoprotein J (apoJ) is synthesized by and found within the human ocular surface epithelium.
  • The localization suggests a functional role for apoJ at the critical tear-ocular surface interface.