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Analytical procedure for the determination of 1-octacosanol in plasma by solvent extraction and capillary gas
L Gonzalez-Bravo1, J Magraner-Hernandez, P C Acosta-Gonzalez
1Center of Natural Products, National Center for Scientific Research, Cubanacan, Havana, Cuba.
Abstract:
A simple and rapid method for the determination of 1-octacosanol in plasma, based on an improved Folch extraction technique and capillary gas chromatography, has been developed taking into account the analytical criteria for the pharmacokinetic studies. The procedure was validated in the range of 50-2000 ng/ml. Despite the complexity of the obtained fingerprints, the efficiency and the separation power of GC allowed the determination of 1-octacosanol in plasma samples. The high recoveries (94.5-98.7%) and precision (1.8-5.8%) obtained are in accordance with the established validation criteria. The validity of this method for pharmacokinetic purposes was shown using an endovenous experiment in animals.