Partial characterization of the silk allergens in mulberry silk extract

Although there have been reports of allergic reactions such as asthma and hypersensitivity pneumonitis to silk allergens (especially in oriental countries), these allergens have not been characterized. We report an in vivo and in vitro study of subjects allergic to silk, as well as a partial characterization of the allergens involved. Forty-one subjects with a clinical history of silk allergy who had asthma, allergic rhinitis or both, and who had a positive intradermal skin test to mulberry silk extract, were evaluated in the study. Four asthmatic subjects with negative skin tests and RASTs to mulberry silk extract were used as controls. RAST specific lgE was present in 37/41 (90%) subjects. SDS-PAGE of mulberry silk extract demonstrated 13 protein bands of less than 21-95 kD. lgE which bound to protein bands was present in 13/32 (41%) sera, twelve of which bound to a single protein with a molecular weight between 35.1 and 50 kD. A pool made with the four control sera did not react with any protein band. The patients allergic to silk showed evidence of sensitivity to mulberry silk extract, both in vivo and in vitro, but sera from 19/32 (59%) subjects did not have antibodies reactive with the mulberry silk extract immunoblot protein bands. The low avidity of the lgE may be responsible for this discrepancy between in vivo and in vitro results.