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Characterization of non-pigmented Staphylococcus chromogenes
1Department of Animal Health II, School of Veterinary Medicine, Azaba University, Sagamihara, Japan.
The Journal of Veterinary Medical Science
|July 1, 1996
Summary
Non-pigmented Staphylococcus chromogenes strains were identified in piglets. These isolates were genetically similar to S. chromogenes but distinct from S. hyicus, despite similar colony appearances.
Area of Science:
- Veterinary Microbiology
- Bacterial Taxonomy
Background:
- Staphylococcus chromogenes is a coagulase-negative staphylococcal species commonly found on animal skin.
- Exudative epidermitis in piglets is a significant disease, often associated with Staphylococcus hyicus infections.
- Pigmentation is a common characteristic of many Staphylococcus species, including S. chromogenes.
Purpose of the Study:
- To characterize non-pigmented Staphylococcus chromogenes isolated from piglets.
- To differentiate these non-pigmented strains from Staphylococcus hyicus based on phenotypic and genotypic characteristics.
Main Methods:
- Isolation and cultivation of bacterial strains from piglet skin.
- Phenotypic characterization including biochemical tests (hyaluronidase, DNase, Tween 80 hydrolysis, bacitracin resistance).
- DNA-DNA hybridization to assess genetic relatedness.
Main Results:
- Non-pigmented S. chromogenes strains were isolated from both diseased and healthy piglets.
- These isolates showed high DNA homology with the S. chromogenes type strain, confirming their identity.
- Phenotypic tests and DNA homology clearly distinguished these strains from S. hyicus, despite morphological similarities.
Conclusions:
- Non-pigmented S. chromogenes can be found on piglet skin and may be misidentified based on colony morphology alone.
- Accurate identification requires a combination of phenotypic testing and genotypic analysis.
- Understanding the prevalence and characteristics of S. chromogenes is important for swine health management.