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Entire nucleotide sequence of mitochondrial DNA of MS/Ae mice
1Itoham Central Research Institute, Ibaraki, Japan.
Abstract:
The entire nucleotide sequence of mitochondrial DNA of MS/Ae mice was determined. It consists of 16,300 bases, with 15 sites being different from the known 16,295 base sequence of mitochondrial DNA derived from L cells of C3H mice (accession no. V00711). The MS/Ae strain is a derivative of CD-1 mice; these 15 sites in mitochondrial DNA from CD-1 mice were also determined. No difference was found, strongly suggesting that mitochondria of MS/Ae and CD-1 mice have the same DNA sequence and indicating that the high sensitivity of MS/Ae mice to mutagens compared to CD-1 mice is not dependent on genes coded by mitochondrial DNA. (The nucleotide sequence data in this article will appear in the DDBJ, EMBL, and GenBank nucleotide sequence database with the following accession number: D83491).
Insights
Mitochondrial DNA (mtDNA) sequences of MS/Ae mice were analyzed, revealing 15 differences from L cell mtDNA. However, MS/Ae and CD-1 mice share identical mtDNA sequences, suggesting mutagen sensitivity isn't linked to mtDNA genes.
Area of Science:
- Genomics
- Mitochondrial Biology
- Toxicology
Background:
- Mitochondrial DNA (mtDNA) plays a crucial role in cellular respiration and is susceptible to mutations.
- The MS/Ae mouse strain exhibits heightened sensitivity to mutagens compared to the CD-1 strain.
- Understanding the genetic basis of this sensitivity is vital for toxicological studies.
Purpose of the Study:
- To determine the complete nucleotide sequence of the mitochondrial DNA (mtDNA) of MS/Ae mice.
- To compare the mtDNA sequence of MS/Ae mice with that of CD-1 mice and L cell-derived mtDNA.
- To investigate whether differences in mtDNA contribute to the differential sensitivity of MS/Ae mice to mutagens.
Main Methods:
- Whole mitochondrial genome sequencing of MS/Ae mice.
- Bioinformatic analysis to compare nucleotide sequences.
- Identification of sequence variations between MS/Ae, CD-1, and L cell mtDNA.
Main Results:
- The complete mtDNA sequence of MS/Ae mice was determined to be 16,300 bases.
- Fifteen nucleotide differences were identified when compared to the L cell (C3H mouse) mtDNA sequence (accession no. V00711).
- No sequence differences were found in the 15 identified sites between MS/Ae mice and CD-1 mice, indicating identical mtDNA sequences.
Conclusions:
- The mitochondrial DNA sequences of MS/Ae and CD-1 mice are identical.
- The high sensitivity of MS/Ae mice to mutagens is not attributable to genetic variations in their mitochondrial DNA.
- Further research should explore nuclear DNA or other factors contributing to the observed mutagen sensitivity in MS/Ae mice.