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Repeated microdialysis perfusions: periprobe tissue reactions and BBB permeability
E C de Lange1, M Danhof, C Zurcher
1Division of Pharmacology, University of Leiden, Netherlands. 1.lange/lacdr.leidenuniv.nl
Brain Research
|December 8, 1995
Summary
Repeated microdialysis procedures can cause brain tissue changes, including increased hypercellularity and granulocyte infiltration. Frequent use may alter blood-brain barrier permeability, impacting intracerebral microdialysis studies.
Area of Science:
- Neuroscience
- Histology
- Biomedical Engineering
Background:
- Intracerebral microdialysis is a technique used to monitor brain chemistry.
- Understanding the tissue response to microdialysis probes is crucial for accurate results.
- Repeated procedures may induce inflammatory responses in brain tissue.
Purpose of the Study:
- To investigate the histological changes in brain tissue following microdialysis probe insertion.
- To evaluate the impact of repeated microdialysis procedures on brain tissue.
Main Methods:
- Semiquantitative histological analysis of brain tissue.
- Examination of tissue reactions to microdialysis probe presence and repeated perfusions.
Main Results:
- Minimal tissue reactions were observed with the initial probe presence.
- More than two repeated perfusion procedures led to increased hypercellularity.
- Repeated procedures resulted in elevated granulocyte infiltration into the brain tissue.
Conclusions:
- Frequent, repeated microdialysis procedures can induce significant brain tissue changes.
- These tissue effects may be associated with alterations in blood-brain barrier (BBB) permeability.
- Researchers must consider these potential tissue effects when applying repeated intracerebral microdialysis in the same animal.