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Related Experiment Videos

CIF, an essential cofactor for TFIID-dependent initiator function

J Kaufmann1, C P Verrijzer, J Shao

  • 1Howard Hughes Medical Institute, University of California, Los Angeles School of Medicine 90095-1662, USA.

Genes & Development
|April 1, 1996
PubMed
Summary
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A newly identified factor, CIF, is essential for initiator (Inr) element activity in mammalian core promoters. This discovery highlights distinct requirements for TATA box and Inr-driven transcription, explaining promoter diversity in protein-coding genes.

Area of Science:

  • Molecular Biology
  • Gene Regulation
  • Biochemistry

Background:

  • Mammalian core promoters utilize TATA boxes and initiator (Inr) elements for gene transcription.
  • The TFIID complex is crucial for both TATA and Inr element activity.
  • The precise factors for TFIID-dependent Inr activity are not fully understood.

Purpose of the Study:

  • To reconstitute Inr activity using purified general transcription factors.
  • To identify factors responsible for TFIID-dependent Inr-mediated transcription.
  • To elucidate the differences between TATA and Inr promoter element requirements.

Main Methods:

  • Reconstitution of Inr activity with purified recombinant general transcription factors.
  • Assaying Inr activity by measuring enhancement of a TATA-containing core promoter.

Related Experiment Videos

  • Complementation assays to identify the factor imparting Inr activity.
  • Functional and immunological characterization of the identified factor (CIF).

    • Main Results:

    • Basal TATA-mediated transcription was reconstituted with TBP or TFIID and other factors, but Inr activity was not observed.
    • A novel factor, CIF (Inr-associated factor), was identified that specifically stimulates Inr activity in the presence of TFIID, but not TBP.
    • CIF was found to contain multiple components, including the mammalian homolog of Drosophila TAF(II)150, which is not tightly bound to TFIID.

    Conclusions:

    • Distinct molecular mechanisms govern basal TATA and TFIID-dependent Inr promoter activities.
    • The identified factor CIF, containing TAF(II)150, plays a critical role in Inr-mediated transcription.
    • Understanding these differences is key to explaining the heterogeneity of core promoter elements in protein-coding genes.