Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Globin RNA precursor molecules: biosynthesis and process in erythroid cells

R N Bastos, H Aviv

    Cell
    |July 1, 1977
    PubMed
    Summary

    Researchers studied globin mRNA production in red blood cells using a pulse-chase technique. They identified larger precursor molecules in the nucleus, revealing a pathway for globin messenger RNA biosynthesis.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Alveolar soft part sarcoma--reciprocal translocation between chromosome 17q25 and Xp11. Report of a case with metastases at presentation and review of the literature.

    Acta orthopaedica Belgica·2003
    Same author

    Age dependent aneuploidy and telomere length of the human vascular endothelium.

    Atherosclerosis·2001
    Same author

    Sex chromosome mosaicism in gonads of a fetus with cystic hygroma and deletion of the short arm of Y chromosome including loss of SRY.

    American journal of medical genetics·2001
    Same author

    Loss of chromosome 13 in cultured human vascular endothelial cells.

    Experimental cell research·2000
    Same author

    Telomere attrition of the human abdominal aorta: relationships with age and atherosclerosis.

    Atherosclerosis·2000
    Same author

    Telomeres and essential hypertension.

    American journal of hypertension·1999

    Area of Science:

    • Molecular Biology
    • Cell Biology
    • Biochemistry

    Background:

    • Globin mRNA is essential for red blood cell function.
    • Understanding mRNA biosynthesis is crucial for cellular processes.

    Purpose of the Study:

    • To investigate the biosynthetic pathway of globin mRNA in erythroid cells.
    • To identify precursor molecules involved in globin mRNA formation.

    Main Methods:

    • Hybridization of labeled RNA with complementary DNA.
    • Utilized a pulse-chase technique.
    • Analyzed RNA species in erythroid cell nuclei and cytoplasm.

    Main Results:

    • Detected three nuclear RNA species sharing sequences with globin mRNA.
    • Identified two larger precursors: a 27S (approx. 5000 nucleotides) and a 15S (approx. 1500 nucleotides) molecule.
    • The 27S species lacked poly(A) sequences, while 15S and 10S species contained them. The 27S precursor rapidly degraded, while the 10S cytoplasmic mRNA accumulated.

    Conclusions:

    • Proposed a model for globin mRNA biosynthesis.
    • The pathway involves transcription of a large 27S precursor, followed by cleavage into a 15S intermediate.
    • Further processing yields the mature 10S globin mRNA for cytoplasmic accumulation.

    Related Experiment Videos