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The cell-coded polypeptide U90 increased by herpes simplex virus type 2 infection induces Fos and DNA synthesis
J C Macnab1, M D Davison, D McNab
1MRC Virology Unit, University of Glasgow, UK.
The Journal of General Virology
|December 1, 1995
Summary
Herpes simplex virus type 2 (HSV-2) produces a protein, U90, that aids viral replication by stimulating cell growth and DNA synthesis. This protein may also promote proliferation in abnormal cells.
Area of Science:
- Virology
- Molecular Biology
- Cell Biology
Background:
- Herpes simplex virus type 2 (HSV-2) lytic infection triggers the synthesis of a 90 kDa cell-coded protein, designated U90.
- U90 expression is specific to tumor cells and is excreted from the infected cell.
Purpose of the Study:
- To investigate the function of U90 and its potential advantages for HSV-2 replication.
- To determine the effects of purified U90 on quiescent fibroblast cell growth.
Main Methods:
- Purification of U90 from the medium of HSV-2 infected cells.
- Characterization of purified U90, confirming similarity to the internalized form.
- Studying the effect of U90 addition on quiescent fibroblast cell growth.
Main Results:
- U90 addition to quiescent fibroblast cells stimulated Fos expression, a product of the cellular transcription factor c-fos.
- U90 increased the incorporation of [3H]thymidine into DNA, suggesting a role in DNA synthesis.
- These effects may facilitate HSV-2 replication.
Conclusions:
- The U90 protein synthesized during HSV-2 lytic infection plays a role in promoting host cell proliferation.
- U90's ability to stimulate Fos expression and DNA synthesis may enhance viral replication.
- There is a potential implication that U90 could induce proliferation in abnormal cells, such as those in precancerous lesions.