[A new quantitative assay of heat shock protein 70 by based on an enzyme-linked immunosorbent assay method]

Heat Shock Protein 70 (HSP70) was very important stress protein. HSP70 had been detected by immunoblotting or mRNA of HSP70. Our purpose is to develop an easy quantitative assay of HSP70 and utilize clinical samples, cells, tissues etc. We examined a new quantitative assay of HSP70 that was based on an enzyme-linked immunosorbent assay (ELISA). This quantitative assay showed reproducible standard curve, high reproducibility (CV : 7.1, 6.8%) and recovery (93 approximately 116%). The determination of HSP70 in human umbilical vein endothelial cells (HUVEC) by heat stress was assayed using this method. HSP70 in HUVEC increased according to the heat temperature. It is concluded that the quantity of HSP70 might be possible even in cells and tissues by this method.