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Updated: Aug 21, 2026

The Use of Gas Chromatography to Analyze Compositional Changes of Fatty Acids in Rat Liver Tissue during Pregnancy
Published on: March 13, 2014
Pretreatment methods prior to gaschromatographic analysis of volatile fatty acids from faecal samples
Abstract:
Vacuum distillation and steam distillation have been compared as pretreatment methods prior to the GC analysis of volatile fatty acids (VFA) in faecal material. Vacuum distillation resulted in a recovery range of 90-110% for all VFA in standard solutions. This was superior to the recovery ranges found after pretreatment by steam distillation. Coefficients of variance for steam distilled samples were higher than for vacuum distilled samples. Using a faecal homogenate, the vacuum distillation method was found to be highly reproducible. Spiking experiments substantiated the quantitative recovery of VFA from vacuum distilled samples. Modifications of the standard vacuum distillation procedure showed that care must be taken to ensure adequate cooling of the receiver tube.
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