Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Fiber-optic DNA sensor for fluorometric nucleic acid determination

P A Piunno1, U J Krull, R H Hudson

  • 1Chemical Sensors Group, Department of Chemistry, Erindale College, Univerity of Toronto, Ontario, Canada.

Analytical Chemistry
|August 1, 1995
PubMed
Summary

Researchers developed a novel optical DNA biosensor using synthesized single-stranded deoxyribonucleic acid (ssDNA) on quartz optical fibers. This regenerable biosensor detects complementary DNA (cDNA) and RNA (cRNA) with high sensitivity and a low detection limit.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Intraethnic comparison of eating attitudes in native Koreans and Korean Americans using a Korean translation of the eating attitudes test.

The Journal of nervous and mental disease·1998
Same author

Pulmonary nodule detection: low-dose versus conventional CT.

Radiology·1998
Same author

A young woman with petechiae.

Lancet (London, England)·1998
Same author

Language development in children with simple-partial left-hemisphere epilepsy.

Brain and language·1998
Same author

Sodium reduction and weight loss for elderly patients with hypertension.

JAMA·1998
Same author

Balance in children with otitis media with effusion.

International journal of pediatric otorhinolaryngology·1998

Area of Science:

  • Biotechnology
  • Biosensor Technology
  • Nucleic Acid Chemistry

Background:

  • Development of sensitive and specific biosensors is crucial for molecular diagnostics.
  • Optical detection methods offer non-invasive and real-time monitoring capabilities.
  • Immobilization of nucleic acids on solid supports is key for creating stable biosensing platforms.

Purpose of the Study:

  • To create a novel optical DNA biosensor utilizing immobilized single-stranded deoxyribonucleic acid (ssDNA) on quartz optical fibers.
  • To evaluate the performance of the biosensor in terms of hybridization efficiency, sensitivity, and regenerability.
  • To demonstrate the potential of the biosensor for detecting complementary nucleic acid sequences.

Main Methods:

  • Synthesis of thymidylic acid icosanucleotides (dT20) on derivatized quartz optical fibers using automated solid-phase synthesis.

Related Experiment Videos

  • Hybridization assays with complementary ssDNA (cDNA) and ssRNA (cRNA) in solution.
  • Detection of hybridization using the fluorescent DNA stain ethidium bromide (EB).
  • Characterization of hybridization extent using UV absorbance thermal denaturation studies.

    • Main Results:

    • The optical DNA biosensor demonstrated successful hybridization with complementary nucleic acid targets.
    • A detection limit of 86 ng/mL cDNA and a sensitivity of 200% fluorescence increase per 100 ng/mL cDNA were achieved.
    • The biosensor proved to be regenerable for at least five cycles and stable after 1 year of storage, harsh washing, and autoclaving.
    • Thermal denaturation studies confirmed the involvement of all 20 bases in duplex formation.

    Conclusions:

    • The developed optical DNA biosensor based on immobilized ssDNA on quartz fibers is a sensitive, specific, and robust platform.
    • The biosensor is regenerable and stable, making it suitable for repeated use and long-term applications.
    • This technology holds promise for various applications in molecular diagnostics and nucleic acid detection.