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Development of a submicrometer optical fiber oxygen sensor

Z Rosenzweig¹, R Kopelman

¹Department of Chemistry, University of Michigan, Ann Arbor 48109, USA.

Analytical Chemistry

|August 1, 1995

Summary

A novel submicrometer optical fiber oxygen sensor utilizes ruthenium(II) complex fluorescence quenching. This highly sensitive and reproducible sensor achieves an unprecedented detection limit for oxygen measurements.

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Area of Science:

Materials Science
Analytical Chemistry
Biomedical Engineering

Background:

Optical fiber sensors offer non-invasive monitoring capabilities.
Accurate dissolved oxygen measurement is crucial in various scientific fields.
Existing optical fiber oxygen sensors face limitations in sensitivity and sample volume.

Purpose of the Study:

To develop a submicrometer optical fiber oxygen sensor with enhanced sensitivity and reproducibility.
To utilize the fluorescence quenching property of a ruthenium(II) complex for oxygen detection.
To minimize reagent leaching and improve sensor stability.

Main Methods:

Fabrication of a submicrometer optical fiber tip.
Covalent attachment of tris-(1,10-phenanthroline)ruthenium(II) chloride within an acrylamide polymer matrix via photoinitiated polymerization.
Optimization of monomer and cross-linker ratios to prevent leaching.
Characterization of sensor performance, including reversibility, reproducibility, and detection limit.

Main Results:

Successful fabrication of a submicrometer optical fiber oxygen sensor.
Minimized leaching of the sensing reagent through polymer matrix optimization.
Achieved high reproducibility with a standard deviation of ~2% for fluorescence measurements.
Demonstrated an absolute detection limit of 1 x 10(-17) mol, a 10^6-fold improvement over existing sensors.
Required sample volume of only 100 fL.

Conclusions:

The developed optical fiber oxygen sensor exhibits superior sensitivity and low detection limits.
The sensor design minimizes reagent leaching, ensuring stability and reproducibility.
This technology offers a significant advancement for dissolved oxygen monitoring in diverse applications.

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