The ultrastructure of the testis in rats after long-term treatment with lead acetate

Studies were performed to investigate the influence of long-term lead acetate treatment on morphology of rat testis. No marked changes were observed by means of light microscopy. At all stages (I-XIV) of the seminiferous epithelium cycle, all generations and layers of spermatogenic cells were present. Electron-microscopic studies did not reveal any ultrastructural changes neither in seminiferous epithelium nor in Sertoli cells. In Leydig cells also, no ultra-structural abnormalities were visible. Macrophages of testicular interstitial tissue contained electron-dense inclusions, usually located inside phagolisosome-like vacuoles. X-ray micro-analysis revealed that the inclusions contained lead.