Related Experiment Video
Updated: Jul 21, 2026

Noninvasive Sampling of Mucosal Lining Fluid for the Quantification of In Vivo Upper Airway Immune-mediator Levels
Published on: August 7, 2017
[Clinical and microbiological study of otitis media in infants]
S Domínguez Rovira1, C Mainou Cid, A Clarós Blanch
1Unidad Integrada Hospital Clínico-San Juan de Dios, Universidad de Barcelona.
Insights
This study found Streptococcus pneumoniae and Haemophilus influenzae are common causes of otitis media in infants. Prompt antibiotic therapy is recommended for all infants diagnosed with this middle ear infection.
Area of Science:
- Pediatrics
- Microbiology
- Infectious Diseases
Context:
- Otitis media is a common childhood infection, particularly in infants.
- Understanding the causative pathogens is crucial for effective treatment and management.
- Previous research indicates bacterial involvement, but local prevalence data is essential.
Purpose:
- To determine the prevalence of bacteria in middle ear effusions of infants diagnosed with otitis media.
- To identify the most common bacterial pathogens responsible for otitis media in this specific infant population.
- To evaluate the role of clinical symptoms and blood analysis in diagnosing infant otitis media.
Summary:
- A prospective study of 50 infants (1 month to 1 year) with otitis media identified pathogens in 62% of ear cultures.
- Streptococcus pneumoniae (38%) and Haemophilus influenzae (25%) were the predominant bacteria found.
- Failure to thrive was a notable presenting symptom (18%), while common symptoms included rhinorrhea, nasal obstruction, cough, and fever.
Impact:
- Findings support the international prevalence of S. pneumoniae and H. influenzae in infant otitis media.
- The study suggests antibiotic therapy should be administered to all infants diagnosed with otitis media.
- Highlights the clinical nature of differentiating acute otitis media from otitis media with effusion, rather than solely microbiological factors.
Abstract:
The objective of this study was to determine the prevalence of bacteria isolated from middle ear effusions in infants with otitis media in our environment. Data collected from 50 patients of the Infant Section of the Unidad Integrada Hospital Clínico-San Juan de Dios were evaluated prospectively from October 1, 1992 to March 7, 1994. Patients between 1 month and 1 year of age, with unilateral or bilateral otitis media diagnosed by otoscopy criteria and positive myringotomy, were recruited for study. Those who had received any antibiotic during the previous 3 days or had been admitted to the hospital more than 5 days before were excluded. We found that failure to thrive (18%) is a common form of onset of otitis media in children under one year of age. Rhinorrhea and nasal obstruction (60%), cough (50%) and fever (46%) were the most frequent symptoms at the moment of diagnosis. Blood analysis does not add any information for diagnosing otitis media. The difference between acute otitis media and otitis media with effusion is likely to be more clinical rather than microbiological. The recovery of pathogens from 62% of the ear cultures correlates with the figures reported in the literature. The predominance of S. pneumoniae (38%), followed by H. influenzae (25%), is in agreement with previous findings internationally. In our study, there is no evidence of viruses alone causing otitis media in infants. Consequently, antibiotic therapy should be indicated in every child with otitis media.
Related Concept Videos
Sputum Studies I: Gram Stain, cytology, and Acid-fast smear and culture
Gram Stain
The Gram Stain is an integral part of sputum studies. It involves the staining of sputum, which permits...
Sputum Studies II: Culture and Sensitivity
Sputum culture and sensitivity is a medical procedure used to diagnose bacterial infections in the respiratory tract and select the most appropriate antibiotics for treatment. This process involves analyzing sputum samples of thick and opaque secretions produced in the lungs and airways. These samples are collected from patients and then sent to the laboratory for analysis.
The test can identify various pathogens responsible for respiratory infections, including Streptococcus,...
Development of Human Microbiota
Microbiome of the Eye
Development of the Oral Microbiota
Microbiota of the Respiratory Tract

