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Characterization of IS1272, an insertion sequence-like element from Staphylococcus haemolyticus

G L Archer1, J A Thanassi, D M Niemeyer

  • 1Department of Microbiology/Immunology, Medical College of Virginia, Virginia Commonwealth University, Richmond 23298-0049, USA. GARCHER@GEMS.VCU.EDU

Antimicrobial Agents and Chemotherapy
|April 1, 1996
PubMed
Summary

A novel insertion sequence element, IS1272, was identified in Staphylococcus haemolyticus. This element is prevalent in methicillin-resistant staphylococcal isolates across multiple species, suggesting its role in antibiotic resistance dissemination.

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Area of Science:

  • Microbiology
  • Genetics
  • Molecular Biology

Background:

  • Methicillin resistance in staphylococci is often linked to deletions in regulatory genes (mecR1 and mecI).
  • Previous work indicated that Staphylococcus haemolyticus isolates can harbor partial deletions in these regulatory genes.

Purpose of the Study:

  • To characterize a novel DNA element found in Staphylococcus haemolyticus.
  • To investigate the distribution and potential role of this element in staphylococcal antibiotic resistance.

Main Methods:

  • DNA sequencing of cloned elements from S. haemolyticus.
  • Database searches for sequence homology.
  • Hybridization studies using DNA probes from the element against genomic DNA from various staphylococcal species.

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Main Results:

  • Identified and sequenced a 1,934-bp insertion sequence element, IS1272, in S. haemolyticus with terminal inverted repeats and two open reading frames.
  • IS1272 is present in multiple intact copies in S. haemolyticus.
  • Incomplete fragments of IS1272 were found in S. aureus and S. epidermidis, with higher prevalence in methicillin-resistant strains.

Conclusions:

  • IS1272 is a mobile genetic element primarily found in S. haemolyticus.
  • IS1272 has disseminated to other staphylococcal species and is associated with methicillin resistance.
  • The element's prevalence in multiresistant isolates suggests a role in the spread of antibiotic resistance.