Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Structural and functional conservation of the Drosophila doublesex splicing enhancer repeat elements

K J Hertel1, K W Lynch, E C Hsiao

  • 1Department of Molecular and Cellular Biology, Harvard University, Cambridge, Massachusetts 02138, USA.

RNA (New York, N.Y.)
|October 1, 1996
PubMed
Summary

Conserved repeat elements in Drosophila doublesex (dsx) splicing enhancers are single-stranded and functionally interchangeable between species. This structure facilitates specific binding by Tra2, crucial for dsx alternative splicing.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Excessive N<sub>2</sub>O consumption due to pipeline leakages detected through integrated financial and carbon accounting.

Canadian journal of anaesthesia = Journal canadien d'anesthesie·2023
Same author

IL-22 relieves sepsis-induced liver injury via activating JAK/STAT3 signaling pathway.

Journal of biological regulators and homeostatic agents·2020
Same author

Gs/Gi Regulation of Bone Cell Differentiation: Review and Insights from Engineered Receptors.

Hormone and metabolic research = Hormon- und Stoffwechselforschung = Hormones et metabolisme·2016
Same author

Effects of ultrasonic processing on degradation of salvianolic acid B in aqueous solution.

Journal of pharmaceutical and biomedical analysis·2016
Same author

Potential rapid solutions to maintain ventilation in the event of anaesthesia machine failure with no access to the patient's airway.

Anaesthesia and intensive care·2016
Same author

Pharmacologic rescue of motor and sensory function by the neuroprotective compound P7C3 following neonatal nerve injury.

Neuroscience·2014

Area of Science:

  • Molecular Biology
  • Developmental Biology
  • Genetics

Background:

  • Alternative splicing of the doublesex (dsx) gene is critical for sexual differentiation in Drosophila.
  • Splicing enhancers regulate alternative splicing by binding specific proteins.
  • The dsx splicing enhancers in different Drosophila species exhibit sequence variations.

Purpose of the Study:

  • To compare the RNA sequences and secondary structures of dsx splicing enhancers from Drosophila melanogaster and Drosophila virilis.
  • To investigate the functional conservation and structural importance of repeat elements within these enhancers.
  • To elucidate the interaction of Transformer (Tra) and Transformer-2 (Tra2) proteins with dsx splicing enhancers.

Main Methods:

  • Comparative sequence and secondary structure analysis of dsx splicing enhancers.

Related Experiment Videos

  • In vitro RNA structure probing using chemical agents.
  • Functional assays of enhancer activity in Tra-/Tra2-dependent splicing.
  • Antisense oligonucleotide inhibition assays.
  • In vitro protein-RNA binding studies with Tra and Tra2.

    • Main Results:

    • dsx splicing enhancers from D. melanogaster and D. virilis share conserved 13-nucleotide repeat elements and single-stranded structures.
    • These repeat elements are functionally interchangeable in in vitro splicing assays.
    • Antisense oligonucleotides targeting the repeats inhibit enhancer activity, highlighting the importance of their single-stranded nature.
    • Tra2 specifically binds to most repeat elements, while Tra shows non-specific binding.

    Conclusions:

    • The conserved sequence and single-stranded structure of dsx splicing enhancer repeats are essential for function.
    • The accessible, single-stranded repeats are recognized by the RNA-binding domain of Tra2, mediating alternative splicing of dsx.
    • These findings provide insights into the molecular mechanisms of alternative splicing regulation in Drosophila sexual development.