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[Plasmid size determination using 3 methods]

A L Darini1, O L Zucchi, G V Carbonell

  • 1Departamento de Análises Clínicas, USP, Brasil.

Revista Latinoamericana De Microbiologia
|July 1, 1995
PubMed
Summary
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Determining bacterial plasmid size is crucial for tracking infections. The power-function method accurately determined plasmid sizes in Enterobacter cloacae, outperforming other mathematical approaches.

Area of Science:

  • Microbiology
  • Molecular Biology
  • Epidemiology

Background:

  • Bacterial plasmid profiles are vital for epidemiological studies, particularly for nosocomial infections.
  • Accurate plasmid size determination is essential for molecular epidemiology.

Purpose of the Study:

  • To compare the accuracy of three mathematical methods for determining plasmid sizes in clinical Enterobacter cloacae samples.
  • To identify the most reliable method for plasmid size estimation within a specific size range.

Main Methods:

  • Agarose gel electrophoresis was used to separate plasmids from clinical Enterobacter cloacae samples.
  • Electrophoretic mobility was compared against seven known-size plasmids (2 kb–100 kb).
  • Three mathematical methods, including power-function and exponential analyses, were evaluated.

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Main Results:

  • The power-function method provided the most accurate plasmid size determinations.
  • Exponential analyses were accurate for plasmids sized 50–100 kb and 2–30 kb.
  • Discrepancies were noted among different methodologies, including a computer software approach.

Conclusions:

  • The power-function method is recommended for accurate bacterial plasmid size determination in the 2 kb–100 kb range.
  • Careful selection of mathematical methods is necessary to avoid discrepancies in plasmid profiling.
  • Standardized methods are needed for reliable molecular epidemiology of bacterial infections.