Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

[Comparison of detection methods for the Staphylococcus aureus capsule]

L Odierno1, A Zandarín, M Ferrari

  • 1Departamento de Microbiología e Inmunología, Universidad Nacional de Río Cuarto, Córdoba, Argentina.

Revista Latinoamericana De Microbiologia
|July 1, 1995
PubMed
Summary

Comparing eleven methods for Staphylococcus aureus capsule detection, researchers found transmission electron microscopy and clumping factor tests most suitable. Clumping factor tests offer rapid presumptive diagnosis for capsulated strains, aiding in identifying Staphylococcus aureus.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Pathogenesis and inflammatory response in experimental caprine mastitis due to Staphylococcus chromogenes.

Microbial pathogenesis·2018
Same author

Spectroscopic identification of the chemical interplay between defects and dopants in Al-doped ZnO.

Physical chemistry chemical physics : PCCP·2017
Same author

Bovine mastitis prevention: humoral and cellular response of dairy cows inoculated with lactic acid bacteria at the dry-off period.

Beneficial microbes·2017
Same author

Staphylococcus aureus avirulent mutant vaccine induces humoral and cellular immune responses on pregnant heifers.

Vaccine·2016
Same author

Zn vacancy induced green luminescence on non-polar surfaces in ZnO nanostructures.

Scientific reports·2014
Same author

Impaired energy expenditure despite normal cardiovascular capacity in children with type 1 diabetes.

Hormone research in paediatrics·2012

Area of Science:

  • Microbiology
  • Bacteriology
  • Molecular Biology

Background:

  • Staphylococcus aureus is an opportunistic pathogen, with capsular polysaccharides playing a crucial role in its virulence and resistance to host defenses.
  • Accurate and efficient detection methods for capsulated Staphylococcus aureus strains are essential for clinical diagnosis and research.
  • Existing detection methods vary in speed, labor intensity, and required resources, necessitating a comparative analysis.

Purpose of the Study:

  • To compare the efficacy and suitability of eleven distinct methods for detecting Staphylococcus aureus capsule production.
  • To identify the most practical and reliable methods for routine laboratory use and specific research applications.
  • To evaluate two methods for inducing capsule production in Staphylococcus aureus.

Main Methods:

Related Experiment Videos

  • Comparative analysis of eleven Staphylococcus aureus capsule detection techniques.
  • Key methods evaluated include transmission electron microscopy, clumping factor determination, serum-soft agar colonial morphology, cell volume estimation, and safranine staining.
  • Two capsule induction methods were tested: in vitro passages in broth with bovine serum and in vitro intraperitoneal inoculation in mice.

Main Results:

  • Transmission electron microscopy, clumping factor determination, serum-soft agar morphology, cell volume estimation, and safranine staining were identified as the most suitable methods.
  • Clumping factor determination is a rapid, effective presumptive test for capsulated strains, requiring confirmation.
  • Cell volume estimation is useful for liquid cultures, safranine staining for genetic studies, and both induction methods successfully promoted capsule production.

Conclusions:

  • Several methods, including clumping factor determination and safranine staining, offer practical and efficient means for detecting Staphylococcus aureus capsules.
  • The choice of method depends on the specific application, such as presumptive diagnosis, liquid culture analysis, or genetic studies.
  • Both in vitro and in vivo induction methods are effective in enhancing Staphylococcus aureus capsule production.