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NADP-malic enzyme isoforms in maize leaves

V G Maurino1, M F Drincovich, C S Andreo

  • 1Centro de Estudios Fotosintéticos y Bioquímicos (CONICET, F. M. Lillo), Rosario, Argentina.

Biochemistry and Molecular Biology International
|February 1, 1996
PubMed
Summary

Maize leaves contain two NADP-malic enzyme isoforms. The 62 kDa form is key for C4 metabolism, while the 72 kDa form, found in etiolated leaves, is less active.

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Area of Science:

  • Plant Biochemistry
  • Molecular Biology
  • Enzymology

Background:

  • NADP-malic enzyme (NADP-ME) is crucial for C4 photosynthesis.
  • Maize exhibits distinct NADP-ME isoforms with varying roles.

Purpose of the Study:

  • To characterize the two NADP-ME isoforms in maize leaves.
  • To investigate their differential expression and relationship.

Main Methods:

  • Biochemical assays (specific activity, Km, pH optimum).
  • Immunological techniques (antibody cross-reactivity).
  • Molecular analyses (Northern and Southern blotting).

Main Results:

  • Two isoforms (72 kDa and 62 kDa) identified with different properties.
  • 62 kDa form predominates in green leaves and increases upon illumination.
  • 72 kDa form is mainly in etiolated leaves and remains constant during greening.
  • Isoforms are immunologically related; multiple genes suggested.

Conclusions:

  • The 62 kDa NADP-ME isoform is central to C4 metabolism in green maize leaves.
  • The 72 kDa isoform, present at low levels in green leaves, may have a distinct or precursor role.
  • Differential expression and relatedness of isoforms elucidated.

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