A glucose biosensor based on an oxygen electrode: in-vitro performances in model buffer solution and in blood plasma

Area of Science:

• Biomedical Engineering
• Electrochemistry
• Enzyme Technology

Background:

• Diabetes mellitus necessitates continuous glucose monitoring for effective management.
• Current monitoring methods present limitations in convenience and continuous data acquisition.

Purpose of the Study:

• To develop and evaluate an implantable biosensor for continuous glucose level monitoring.
• To assess the sensor's performance, reproducibility, and long-term stability in various conditions.

Main Methods:

• Development of an amperometric oxygen electrode-based biosensor utilizing immobilized glucose oxidase.
• Employing a liquid suspension for enzyme material and a rechargeable sensor design.
• Utilizing polycarbonate glucose-diffusion membranes coated with silastic for sensor construction.
• Conducting in vitro calibration and long-term stability tests in phosphate buffer and blood plasma at different temperatures.

Main Results:

• Successful calibration of the biosensor in both phosphate buffer and undiluted blood plasma.
• Demonstrated reproducibility of sensor responses in blood plasma at body temperature.
• Achieved a minimum operational lifetime of six weeks during continuous in vitro testing at body temperature.

Conclusions:

• The developed implantable biosensor provides a viable platform for continuous glucose monitoring.
• The sensor's rechargeable nature and extended lifetime offer significant advantages for diabetic patient care.
• Further in vivo studies are warranted to confirm clinical efficacy and safety.