Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

The cold-shock response in bacteria

A P Wolffe1

  • 1Section on Molecular Biology, National Institute of Child Health and Human Development, NIH, Bethesda, Maryland 20892, USA.

Science Progress
|January 1, 1995
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Purification of the MeCP2/histone deacetylase complex from Xenopus laevis.

Methods in molecular biology (Clifton, N.J.)·2003
Same author

Targeted regulation of imprinted genes by synthetic zinc-finger transcription factors.

Gene therapy·2003
Same author

Profiling methyl-CpG specific determinants on transcriptionally silent chromatin.

Molecular biology reports·2002
Same author

Transcriptional regulation in the context of chromatin structure.

Essays in biochemistry·2002
Same author

Analysis of chromatin-immunopurified MeCP2-associated fragments.

Biochemical and biophysical research communications·2001
Same author

Gene-selective developmental roles of general transcription factors.

Trends in biochemical sciences·2001
Same journal

Gene expression in visceral adipocytes in metabolically healthy and unhealthy obesity: A cross-sectional analysis of associations with cardiometabolic components.

Science progress·2026
Same journal

Proteomic profiling reveals mitochondrial metabolic alterations in dexamethasone-induced neuronal differentiation.

Science progress·2026
Same journal

Stroke risk associated with the interaction between composite dietary antioxidant index and heavy metals: A cross-sectional explainable machine learning study using NHANES data.

Science progress·2026
Same journal

Neuroimaging in schizophrenia: From group-average abnormalities to individualised circuit models.

Science progress·2026
Same journal

Clinical and mechanistic effects of GLP-1 receptor agonists in hidradenitis suppurativa and comorbidities.

Science progress·2026
Same journal

Association between serum albumin-to-globulin ratio and diabetic retinopathy: A cross-sectional study based on the 2001-2020 NHANES database.

Science progress·2026
See all related articles

Escherichia coli

Area of Science:

  • Microbiology
  • Molecular Biology
  • Genetics

Background:

  • The 'cold shock' response in Escherichia coli involves a significant decrease in protein synthesis upon temperature downshift.
  • This response is characterized by the production of specific 'cold shock' proteins that enable growth at low temperatures.
  • A key regulator is the cold shock protein CS7.4, whose synthesis is dramatically induced at low temperatures.

Purpose of the Study:

  • To investigate the role of CS7.4 in the bacterial cold shock response.
  • To explore the evolutionary conservation and functional relationship between bacterial CS7.4 and eukaryotic Y-box proteins.
  • To elucidate the molecular mechanisms underlying cold shock gene regulation in bacteria and eukaryotes.

Main Methods:

  • Comparative sequence analysis of CS7.4 and Y-box proteins.

Related Experiment Videos

  • Studies on the regulation of CS7.4 synthesis during temperature downshift.
  • Investigation of the nucleic acid binding properties of CS7.4 and related eukaryotic proteins.

    • Main Results:

    • CS7.4 exhibits remarkable sequence conservation with the nucleic acid binding domain of eukaryotic Y-box proteins.
    • The induction of CS7.4 synthesis is a central regulatory event in the bacterial cold shock response.
    • Studies revealed conserved molecular mechanisms for cold shock adaptation and gene regulation.

    Conclusions:

    • CS7.4 acts as a crucial cold shock protein in Escherichia coli.
    • The conserved structure suggests a shared ancestral function between bacterial cold shock proteins and eukaryotic Y-box proteins.
    • This research provides insights into novel regulatory sites for gene expression in eukaryotes.