Related Experiment Videos
Chinese Han population study of the D1S80 (pMCT118) locus polymorphisms
B Cong1, N Harashima, Y Katsuyama
1Department of Forensic Medicine, Hebei Medical College, Shijiazhuang, China.
Abstract:
The Chinese Han population was studied for the D1S80 (pMCT118) locus. Bloodstain samples of 216 unrelated individuals were tested through Chelex extraction and the polymerase chain reaction (PCR). After amplification, the genotypes were separated by polyacrylamide gel (PAG) electrophoresis and stained with ethidium bromide. A total of 70 genotypes and 25 alleles were distinguished. Alleles 18, 24 and 30 were the most common alleles in Chinese population. The distribution of the observed genotypes conformed to the Hardy-Weinberg equilibrium for the D1S80 (pMCT118) system. The chance of exclusion, the discriminating power (DP), and the heterozygosity for this system were 0.71, 0.90, and 0.85, respectively, in Chinese population.