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Updated: Aug 12, 2026
06:25
Chromosomics: Detection of Numerical and Structural Alterations in All 24 Human Chromosomes Simultaneously Using a Novel OctoChrome FISH Assay
Published on: February 6, 2012
[The perspective on chromosomal painting (FISH)]
1Department of Pathology, Iwate Medical University, Morioka.
Rinsho Byori. the Japanese Journal of Clinical Pathology
|February 1, 1996
Summary
Fluorescence in situ hybridization (FISH) offers advanced molecular biology techniques for diagnosing genetic and neoplastic diseases. This review covers FISH methods and their clinical applications in laboratory medicine.
Area of Science:
- Molecular Biology
- Cytogenetics
- Laboratory Medicine
Context:
- Sophisticated molecular biology technologies have emerged.
- Fluorescence in situ hybridization (FISH) is a key technique.
- FISH is utilized in both research and clinical diagnostics.
Purpose:
- To review representative FISH techniques.
- To discuss FISH applications in laboratory medicine, including genetic and neoplastic disease diagnosis.
Summary:
- Standard FISH involves whole chromosome painting for structural abnormalities and centromere staining for numerical aberrations.
- Comparative genomic hybridization (CGH) detects gene amplification/deletion in tumors.
- FISH aids in identifying genes involved in neoplasm development.
Impact:
- FISH enables precise diagnosis of genetic disorders.
- FISH supports the identification of chromosomal abnormalities in neoplastic diseases.
- Advanced FISH techniques like CGH facilitate cancer research and targeted therapy development.
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