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Cosmids and transcribed sequences from chromosome 11q23
M Katoh1, Y Nakagawa, T Yawata
1Department of Molecular and Cell Genetics, School of Life Sciences, Tottori University, Japan.
The Japanese Journal of Human Genetics
|December 1, 1995
Summary
Radiation-reduced hybrids (RHs) were used to create DNA markers for human chromosome 11. This method successfully identified specific DNA sequences and genes in the 11q23 region.
Area of Science:
- Genetics
- Molecular Biology
- Genomics
Background:
- Developing region-specific DNA markers is crucial for gene mapping and positional cloning.
- Human chromosome 11 harbors genes associated with various genetic disorders.
- Radiation hybrid mapping offers a method for physical mapping of genes.
Purpose of the Study:
- To generate cosmid markers and identify transcribed sequences from specific regions of human chromosome 11.
- To develop tools for the positional cloning of genes in the 11q23 region.
Main Methods:
- Preparation of radiation-reduced hybrids (RHs) from microcell hybrids containing human chromosome 11.
- Construction of a cosmid library from an RH clone (RH(11)-9) containing the 11q23 fragment.
- Screening and localization of cosmid clones using Southern hybridization with an RH panel.
- Exon amplification from selected cosmid clones.
Main Results:
- Fifteen RH clones were isolated, with RH(11)-9 containing the 11q23 fragment.
- Fifty-nine cosmids were assigned to 11q23 and 6 to 11p11.2.
- Sixteen putative exons were cloned, including sequences from the known ATDC gene on 11q23.
Conclusions:
- Radiation-reduced hybrids are effective for creating region-specific DNA markers on human chromosome 11.
- The identified cosmid markers and putative exons facilitate gene discovery and positional cloning in the 11q23 region.