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Characterization of a single-locus minisatellite DNA in Xenopus laevis
1Department of Biochemistry and Molecular Biology, Colorado State University, Fort Collins 80523, USA.
Genome
|February 1, 1996
Abstract:
We have cloned a minisatellite tandem array (XTA) from Xenopus laevis that contains approximately 200 copies of the 20-bp repeat 5'-CCAACAGCCTGCCCATCCAT-3'. The XTA sequence is present only once per haploid genome and is polymorphic with respect to repeat number and location of flanking restriction endonuclease sites. Although the 20-bp repeat has not previously been described, flanking sequences suggest that it lies proximal to coding regions in the Xenopus genome.