Sensitive high-performance thin-layer chromatography method for detection and determination of ranitidine in plasma...

Area of Science:

• Analytical Chemistry
• Pharmacology

Background:

• Ranitidine is a widely used H2-receptor antagonist.
• Accurate quantification of ranitidine in biological matrices like plasma is essential for pharmacokinetic and therapeutic drug monitoring.
• Existing methods may require complex procedures or internal standards.

Purpose of the Study:

• To develop and validate a high-performance thin-layer chromatographic (HP-TLC) method for ranitidine determination in human plasma.
• To establish a rapid and reliable quantification technique that does not necessitate the use of internal standards.

Main Methods:

• High-performance thin-layer chromatography (HP-TLC) was employed.
• A single-stage extraction procedure was utilized for ranitidine isolation from plasma.
• Quantification was performed using a Shimadzu CS930 dual-wavelength TLC scanner.
• Precoated silica gel F254 plates were used for chromatographic separation.

Main Results:

• The developed HP-TLC method demonstrated high performance for ranitidine determination.
• The procedure allowed for accurate quantification without the need for internal standards.
• A direct estimate of total ranitidine concentration in plasma was achieved.

Conclusions:

• The established HP-TLC method is a sensitive and efficient tool for ranitidine analysis in plasma.
• This technique simplifies the quantification process by eliminating the requirement for internal standards.
• The method provides a direct and reliable measure of ranitidine, valuable for clinical and research applications.