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Antibiotic sensitivity of Staphylococcus aureus and Staph. intermedius of canine and feline origin
1Department of Biology, Trinity Western University, Langley British Columbia, Canada.
Abstract:
Disc agar diffusion testing was performed on 547 isolates (two common pathogens) to determine if the site of isolation influenced the antimicrobial susceptibility results for a given bacterium. The most statistically significant results (P < 0.05) included cephalothin (ear) against Staphylococcus aureus and cephalothin (ear), lincomycin (ear), trimethoprim sulpha (ear), and amoxycilin and clavulanic acid (nose) against Staph. intermedius. Although the impact of these results (empirical treatment) is unknown, it is hypothesized that the site of isolation of Staph. aureus and Staph. intermedius may influence the choice of antimicrobial therapy in the dog and cat.
Insights
The isolation site of Staphylococcus aureus and Staphylococcus intermedius may affect antimicrobial susceptibility. This finding could influence empirical treatment choices for canine and feline bacterial infections.
Area of Science:
- Veterinary Microbiology
- Antimicrobial Resistance
- Clinical Pathology
Background:
- Staphylococcus aureus and Staphylococcus intermedius are common pathogens in dogs and cats.
- Antimicrobial susceptibility testing is crucial for guiding effective treatment.
- The influence of isolation site on susceptibility patterns is not well-established.
Purpose of the Study:
- To investigate whether the anatomical site of isolation impacts antimicrobial susceptibility results for Staphylococcus aureus and Staphylococcus intermedius.
- To identify specific antimicrobial agents and isolation sites with statistically significant differences in susceptibility.
Main Methods:
- Disc agar diffusion testing was performed on 547 bacterial isolates.
- Isolates were identified as Staphylococcus aureus or Staphylococcus intermedius.
- Data were analyzed to determine statistically significant correlations between isolation site and antimicrobial susceptibility (P < 0.05).
Main Results:
- Significant differences in susceptibility were observed based on isolation site.
- Staphylococcus aureus showed varying susceptibility to cephalothin when isolated from the ear.
- Staphylococcus intermedius exhibited differential susceptibility to cephalothin, lincomycin, trimethoprim sulfa, and amoxicillin-clavulanic acid depending on ear or nose isolation.
Conclusions:
- The anatomical site of isolation for Staphylococcus aureus and Staphylococcus intermedius may influence antimicrobial susceptibility.
- These findings suggest that the site of infection could be a factor in selecting empirical antimicrobial therapy for companion animals.
- Further research is needed to determine the clinical impact of these site-specific susceptibility variations on empirical treatment outcomes.