Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Selective immunomodulation: utilization of CD29/VLA molecules

C Morimoto1, T Sato, K Tachibana

  • 1Dana-Farber Cancer Institute, Department of Medicine, Harvard Medical School, Boston, MA 02115, USA.

Artificial Organs
|August 1, 1996
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Anti-inflammatory Annexin A1 in Periodontitis via Formyl Peptide Receptor 2.

Journal of dental research·2025
Same author

[Clinical analysis of fertility-sparing therapy of patients with complex atypical hyperplasia and endometrial cancer].

Beijing da xue xue bao. Yi xue ban = Journal of Peking University. Health sciences·2022
Same author

Clinical impacts of sun exposures on the faces and hands of Japanese women of different ages.

International journal of cosmetic science·2019
Same author

Hypoxia-inducible factor-1α inhibits interleukin-6 and -8 production in gingival epithelial cells during hypoxia.

Journal of periodontal research·2016
Same author

Regulation of somatostatin receptor 4-mediated cytostatic effects by CD26 in malignant pleural mesothelioma.

British journal of cancer·2014
Same author

Role of the β1 integrin molecule in T-cell activation and migration.

Modern rheumatology·2014
Same journal

Large-Eddy Simulation of the FDA Benchmark Blood Pump: Validation Against Experiments and Implications for Turbulent Flow Mechanisms.

Artificial organs·2026
Same journal

The Warm Revolution: A Meta-Analysis of DCD Versus DBD Liver Transplant Outcomes in the Normothermic Machine Perfusion Era.

Artificial organs·2026
Same journal

Toward Optimal Remote Monitoring in LVAD Recipients: Remaining Challenges Beyond Feasibility.

Artificial organs·2026
Same journal

Advancing Organ Preservation and Perfusion: Introducing the International Society of Organ Preservation and Perfusion Therapy (ISOPPT).

Artificial organs·2026
Same journal

Short Inter-Treatment Interval Treatment With Artificial Liver Support System Reduces 90-Day Transplant-Free Mortality in Patients With Hepatitis B Virus-Related Acute-On-Chronic Liver Failure: A Retrospective Observational Study.

Artificial organs·2026
Same journal

Extracorporeal Albumin Dialysis (OPAL) as Novel Therapeutic Bridging Option in Posthepatectomy Liver Failure.

Artificial organs·2026
See all related articles

Researchers developed a bispecific antibody-toxin conjugate targeting CD4+CD29+ memory T cells, crucial in graft rejection. This approach aims for selective immunosuppression in organ transplantation by targeting these specific T cells.

Area of Science:

  • Immunology
  • Molecular Biology
  • Cell Biology

Background:

  • CD4+CD29+ memory T cells are key in graft rejection.
  • VLA/CD29 molecule is vital for T-cell costimulation.
  • Targeting specific T-cell populations is crucial for selective immunosuppression.

Purpose of the Study:

  • To develop a bispecific antibody-toxin conjugate targeting CD4+CD29+ memory T cells.
  • To investigate the molecular mechanisms of VLA-4 crosslinking-induced signaling pathways.
  • To explore novel drug design for selective immunosuppression in organ transplantation.

Main Methods:

  • Preparation of a bispecific antibody-toxin conjugate.
  • Solid-phase crosslinking of VLA-4 using antibodies or fibronectin.
  • Analysis of tyrosine phosphorylation of cellular proteins.

Related Experiment Videos

  • Identification of phosphorylated proteins using molecular weight and binding assays.
  • Site-directed mutagenesis to study protein-protein interactions.

    • Main Results:

    • A bispecific antibody-toxin conjugate targeting CD4+CD29+ memory T cells was successfully prepared.
    • VLA-4 crosslinking stimulated tyrosine phosphorylation of multiple proteins, including PLC gamma, pp125FAK, paxillin, Fyn, Lck, and MAP kinase.
    • pp125FAK was found to be directly associated with paxillin.
    • The paxillin-binding domain of pp125FAK is homologous to that of vinculin, and mutations abolish binding.

    Conclusions:

    • The developed bispecific antibody-toxin conjugate offers a potential strategy for selective immunosuppression.
    • Understanding VLA-4 signaling pathways provides insights into T-cell activation and graft rejection.
    • This system holds promise for novel drug design in organ transplantation, targeting specific T-cell populations.