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Spatial and temporal changes of type VI collagen expression during mouse development

D Marvulli1, D Volpin, G M Bressan

  • 1Institute of Histology and Embryology, University of Padova, Italy.

Developmental Dynamics : an Official Publication of the American Association of Anatomists
|August 1, 1996
PubMed
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Type VI collagen mRNA expression peaks during early mouse development and is prominent in mesenchymal cells, particularly in connective tissues. This suggests a role in defining tissue boundaries.

Area of Science:

  • Molecular Biology
  • Developmental Biology
  • Biochemistry

Background:

  • Type VI collagen is a crucial extracellular matrix protein involved in tissue structure and integrity.
  • Understanding the spatiotemporal expression patterns of collagen chains is vital for comprehending developmental processes and tissue organization.

Purpose of the Study:

  • To investigate the expression patterns of alpha 1 (VI) collagen mRNA and protein during mouse embryonic and postnatal development.
  • To elucidate the cellular sources and tissue distribution of type VI collagen.
  • To explore the potential role of type VI collagen in connective tissue organization.

Main Methods:

  • Northern blotting was employed to detect alpha 1 (VI) collagen mRNA levels in whole mouse embryos and specific adult tissues.

Related Experiment Videos

  • In situ hybridization was used to localize alpha 1 (VI) collagen mRNA in embryonic and developing tissues.
  • Immunocytochemistry was utilized to determine the distribution of type VI collagen protein.

    • Main Results:

    • Alpha 1 (VI) collagen mRNA expression increased significantly from embryonic day 10.5, peaked at birth, and declined in adults.
    • Mesenchymal cells were identified as the primary producers of alpha 1 (VI) collagen mRNA in developing embryos.
    • Type VI collagen protein and mRNA were found in various connective tissues, notably around skeletal muscle, joints, and beneath epithelia, but absent in epithelia and the central nervous system.

    Conclusions:

    • Type VI collagen is dynamically expressed during mouse development, with peak production coinciding with rapid growth phases.
    • Mesenchymal cells are the main source of type VI collagen, which is preferentially deposited in the pericellular environment.
    • The distinct distribution pattern suggests type VI collagen plays a role in delineating boundaries within connective tissues.