Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Analysis of vaccine stability

D B Volkin1, C J Burke, G Sanyal

  • 1Department of Vaccine Pharmaceutical Research, Merck Research Laboratories, West Point, PA, USA.

Developments in Biological Standardization
|January 1, 1996
PubMed
Summary

Vaccine formulation is complex due to labile components. This study explores empirical and rational strategies, including biophysical techniques, to enhance vaccine stability and ensure immunogenicity for global distribution.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

When the individual comes into play: The role of self and the partner in the dyadic play fighting of rats.

Behavioural processes·2023
Same author

Who's laughing? Play, tickling and ultrasonic vocalizations in rats.

Philosophical transactions of the Royal Society of London. Series B, Biological sciences·2022
Same author

Measuring Play Fighting in Rats: A Multilayered Approach.

Current protocols·2022
Same author

The Use of a GroEL-BLI Biosensor to Rapidly Assess Preaggregate Populations for Antibody Solutions Exhibiting Different Stability Profiles.

Journal of pharmaceutical sciences·2017
Same author

Adaptive Value Normalization in the Prefrontal Cortex Is Reduced by Memory Load.

eNeuro·2017
Same author

Perforation of gastrointestinal tract by poorly conspicuous ingested foreign bodies: radiological diagnosis.

The British journal of radiology·2015

Area of Science:

  • Vaccinology
  • Biopharmaceutical Formulation
  • Protein Chemistry

Background:

  • Vaccines comprise diverse antigenic components and excipients, requiring careful formulation to maintain integrity and immunogenicity.
  • The inherent instability of vaccines poses challenges for global distribution, administration, and efficacy, particularly in regions lacking cold chain infrastructure.
  • Factors like combination vaccines and oral delivery can further compromise vaccine stability.

Purpose of the Study:

  • To review strategies for stabilizing vaccines, addressing challenges in maintaining chemical and structural integrity.
  • To explore both empirical screening and rational approaches for identifying effective vaccine stabilizers and optimal environmental conditions.
  • To investigate the application of classical and biophysical techniques for characterizing vaccine physicochemical stability.

Main Methods:

  • Review of empirical methods, including viral plaque assays, for identifying vaccine stabilizers.
  • Application of hydrodynamic and spectroscopic biophysical techniques to assess vaccine stability.
  • Characterization of physicochemical stability for purified Hepatitis A and B vaccine preparations.

Main Results:

  • Classical techniques like viral plaque assays can empirically identify stabilizers.
  • Biophysical methods offer insights into the physicochemical stability of vaccine components.
  • Understanding inactivation pathways enables rational stabilization strategies.

Conclusions:

  • Vaccine stabilization requires a combination of empirical testing and rational design based on understanding inactivation mechanisms.
  • Biophysical techniques are valuable tools for characterizing vaccine stability and guiding formulation development.
  • Improved vaccine stability is crucial for ensuring effective global immunization programs.

Related Experiment Videos