Dependence of cell yield and specific growth rate of Candida boidinii on temperature during continuous cultivation

Investigation of the dependence of cell yield of Candida boidinii in a chemostat and specific growth rate in a turbidostat on temperature was carried out using methanol as substrate. Each of the curves obtained had only one maximum, the optimum temperature for yield (Ys) and for specific growth rate (micron) being somewhat different. With deviation of temperature from the corresponding optimum the micron value decreases more sharply than that of Ys. The shape of two-factor dependences Ys(micron, T) and micron(S, T) is analyzed on the basis of these data and the known dependences of Ys on micron and micron on the limiting substrate concentration S. It is shown that the decelerating action of methanol on the growth rate increases at elevated and lower temperatures.