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Scanning force microscopy of biological samples

M Lekka¹, J Lekki, A P Shoulyarenko

¹Niewodniczański Institute of Nuclear Physics, Kraków.

Polish Journal of Pathology : Official Journal of the Polish Society of Pathologists

|January 1, 1996

Summary

The scanning force microscope (SFM) effectively images 3D biological surfaces in air and liquid. This technique visualizes diverse specimens, including red blood cells and macrophages, showcasing its versatility.

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Area of Science:

Biophysics
Materials Science
Cell Biology

Background:

High-resolution imaging of biological surfaces is crucial for understanding cellular function and disease.
Existing imaging techniques may have limitations in sample preparation or environmental conditions.

Purpose of the Study:

To demonstrate the capability of the scanning force microscope (SFM) for three-dimensional surface imaging of biological specimens.
To showcase the application of SFM in both air and liquid environments.

Main Methods:

Utilized a scanning force microscope (SFM) setup for surface imaging.
Acquired topographical data of biological samples under different environmental conditions (air and liquid cell).

Main Results:

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Successfully obtained three-dimensional surface images of various biological samples, including red blood cells (RBCs), lymphocytes, pronase aggregates, bone surfaces, and macrophage surfaces.
Demonstrated imaging of RBCs, lymphocytes, pronase, and bone in air.
Showcased imaging of macrophage surfaces in a phosphate-buffered saline (PBS) solution.

Conclusions:

The SFM is a versatile tool for high-resolution 3D surface imaging of diverse biological specimens.
SFM can effectively image biological samples in both air and physiological liquid environments, expanding its applicability in life sciences research.