Related Experiment Video

Updated: Aug 23, 2026

Quantification of Proteins Using Peptide Immunoaffinity Enrichment Coupled with Mass Spectrometry
06:09

Quantification of Proteins Using Peptide Immunoaffinity Enrichment Coupled with Mass Spectrometry

Published on: July 31, 2011

[Analysis of chemically synthesized peptides by liquid secondary ion mass spectrometry (LSIMS)]

T Yagami, M Sato, A Nakamura

    Eisei Shikenjo Hokoku. Bulletin of National Institute of Hygienic Sciences
    |January 1, 1994
    PubMed

    More Related Videos

    Quantification of Site-specific Protein Lysine Acetylation and Succinylation Stoichiometry Using Data-independent Acquisition Mass Spectrometry12:49

    Quantification of Site-specific Protein Lysine Acetylation and Succinylation Stoichiometry Using Data-independent Acquisition Mass Spectrometry

    Published on: April 4, 2018

    Sample Preparation and Relative Quantitation using Reductive Methylation of Amines for Peptidomics Studies08:00

    Sample Preparation and Relative Quantitation using Reductive Methylation of Amines for Peptidomics Studies

    Published on: November 4, 2021

    Related Concept Videos

    Electrospray Ionization (ESI) Mass Spectrometry01:12

    Electrospray Ionization (ESI) Mass Spectrometry

    Higher molecular weight biomolecules are nonvolatile compounds that may decompose before ionizing or vaporizing during mass analysis with conventional electron impact ionization methods. Accordingly, electrospray ionization (ESI) is the favored method for vaporizing and ionizing biomolecules as it circumvents rapid fragmentation and enables the recording of mass signals for the entire biomolecule.
    ESI utilizes electrical energy to transfer ions from the liquid phase of the sample into the...
    MALDI-TOF Mass Spectrometry01:19

    MALDI-TOF Mass Spectrometry

    Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.Matrix-assisted laser desorption ionization (MALDI) is a commonly...
    Peptide Identification Using Tandem Mass Spectrometry01:33

    Peptide Identification Using Tandem Mass Spectrometry

    Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
    This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
    Mass Spectrometry: Complex Analysis01:21

    Mass Spectrometry: Complex Analysis

    Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
    GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
    Matrix-Assisted Laser Desorption Ionization (MALDI)01:08

    Matrix-Assisted Laser Desorption Ionization (MALDI)

    Matrix-assisted laser desorption ionization (MALDI) is a powerful analytical technique used in mass spectrometry. It enables the identification and characterization of various biomolecules, including proteins, peptides, nucleic acids, and carbohydrates. MALDI is an ionization technique, widely employed in biological and medical research, as well as in fields like pharmacology and biochemistry.The analyte of interest, a biomolecule or a mixture of biomolecules, is mixed with a suitable matrix...