Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Analyte and label binding assay read by flow cytometry

J O Utgaard1, J Frengen, T Stigbrand

  • 1Department of Physics, Norwegian University of Science and Technology, Trondheim.

Clinical Chemistry
|October 1, 1996
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Exploring novel genomic biomarkers for response and survival after neoadjuvant chemotherapy and radical cystectomy of muscle-invasive bladder cancer.

ESMO open·2025
Same author

Combined treatment with pemetrexed and vinflunine in patients with metastatic urothelial cell carcinoma after prior platinum-containing chemotherapy - results of an exploratory phase I study.

Investigational new drugs·2017
Same author

Monte Carlo study of in-field and out-of-field dose distributions from a linear accelerator operating with and without a flattening-filter.

Medical physics·2012
Same author

Targeting of distinct signaling cascades and cancer-associated fibroblasts define the efficacy of Sorafenib against prostate cancer cells.

Cell death & disease·2012
Same author

Contralateral breast doses measured by film dosimetry: tangential techniques and an optimized IMRT technique.

Physics in medicine and biology·2009
Same author

Current status of prognostic immunohistochemical markers for urothelial bladder cancer.

Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine·2008
Same journal

Comparison of Information-Dependent Acquisition and Sequential Window Acquisition of All Theoretical Mass Spectra for Untargeted Drug Testing on a Linear Ion Trap-Pulsing Quadrupole-Time of Flight Mass Spectrometer.

Clinical chemistry·2026
Same journal

Patterns of One-Year Change in HbA1c and Continuous Glucose Monitoring (CGM) Metrics in Older Adults with Type 2 Diabetes.

Clinical chemistry·2026
Same journal

TSH Pediatric Reference Intervals: Lack of CALIPER Applicability to US-Based Populations.

Clinical chemistry·2026
Same journal

Rapid Detection of Hemoglobinopathy Variants Using One-Step Library Preparation and Nanopore Sequencing.

Clinical chemistry·2026
Same journal

Editor's Note: Circulating Proteolytic Products of Carboxypeptidase N for Early Detection of Breast Cancer.

Clinical chemistry·2026
Same journal

In Reply to Reflexing NT-proBNP for sFlt-1/PlGF Ratios That Fall into the Measurement Uncertainty for Preeclampsia Risk Classification.

Clinical chemistry·2026
See all related articles

A novel assay uses a label-scavenging partner to accurately measure analyte concentration, overcoming hook effect limitations. This method provides reliable results even at high concentrations.

Area of Science:

  • Biochemistry
  • Immunology
  • Analytical Chemistry

Background:

  • The hook effect can limit the accuracy of traditional sandwich immunoassays at high analyte concentrations.
  • Accurate quantification of analytes is crucial in various diagnostic and research applications.

Purpose of the Study:

  • To introduce a new immunometric two-site sandwich assay designed to overcome the hook effect.
  • To enable unambiguous analyte concentration determination, even at high levels.

Main Methods:

  • Incorporation of a label-scavenging binding partner alongside the analyte-binding partner in the assay.
  • Simultaneous generation of two calibration curves using both binding partners.
  • Development of two-particle immunofluorometric assays for placental alkaline phosphatase and human chorionic gonadotropin.

Related Experiment Videos

Main Results:

  • The assay provides a signal proportional to excess label antibody, enabling accurate measurements.
  • A combination of signals from both partners yields unambiguous analyte concentration determination.
  • Assays for placental alkaline phosphatase and human chorionic gonadotropin demonstrated rapid results (2 hours) and wide working ranges (over 5 and 6 orders of magnitude, respectively).

Conclusions:

  • The novel assay effectively eliminates the hook effect, improving accuracy.
  • This method offers a robust solution for quantifying analytes across a broad concentration range.
  • The developed immunofluorometric assays are efficient and highly sensitive for specific biomarker detection.